INSTRUMENTATION + CHEMISTRIES

We provide the following instrumentation, chemistries and primers for our users:

  • Applied Biosystems 3730S 48-capillary DNA Analyzer (x2), with 50cm array and POP-7
  • BigDye® Terminator v3.1 Sequencing Chemistry

Alternative chemistries available for “difficult templates”

  • dGTP BigDye® Terminator v3.0
  • Additives (Betaine or DMSO)

Standard Sequencing Primers

SBC stocks the following STANDARD SEQUENCING PRIMERS, complimentary for SBC-Prepared reactions:

Primer Sequence (5’ – 3’)

-21M13 
TGT AAA ACG ACG GCC AGT

M13R 
CAG GAA ACA GCT ATG ACC

T3 
ATT AAC CCT CAC TAA AGG GA

T7 
TAA TAC GAC TCA CTA TAG GG

T7 Term 
GCT AGT TAT TGC TCA GCG G

SP6 
ATT TAG GTG ACA CTA TAG

pGEX 3’ 
CCG GGA GCT GCA TGT GTC AGA GG

pGEX 5’ 
GGG CTG GCA AGC CAC GTT TGG TG

BGH 
TAG AAG GCA CAG TCG AGG

Custom primers must be submitted at a concentration of 5pmol/µl, resuspended in Tris or dH2O (NOT EDTA)