We provide the following instrumentation, chemistries and primers for our users:
- Applied Biosystems 3730xl 96-capillary DNA Analyzer, with 50 cm array and POP-7 polymer
- BigDye® Terminator v3.1 Sequencing Chemistry
Alternative chemistries available for “difficult templates”
- dGTP BigDye® Terminator v3.0
- Additives (Betaine or DMSO)
Standard Sequencing Primers
SBC stocks the following standard sequencing primers, complimentary for SBC_Core-Prepared reactions:
Custom Sequencing Primers
Custom primers must be submitted at a concentration of 5pmol/µl, resuspended in Tris or dH2O (NO EDTA)