We provide the following instrumentation, chemistries and primers for our users:
- Applied Biosystems 3730S 48-capillary DNA Analyzer (x2), with 50cm array and POP-7
- BigDye® Terminator v3.1 Sequencing Chemistry
Alternative chemistries available for “difficult templates”
- dGTP BigDye® Terminator v3.0
- Additives (Betaine or DMSO)
Standard Sequencing Primers
SBC stocks the following STANDARD SEQUENCING PRIMERS, complimentary for SBC-Prepared reactions:
Primer Sequence (5’ – 3’)
-21M13
TGT AAA ACG ACG GCC AGT
M13R
CAG GAA ACA GCT ATG ACC
T3
ATT AAC CCT CAC TAA AGG GA
T7
TAA TAC GAC TCA CTA TAG GG
T7 Term
GCT AGT TAT TGC TCA GCG G
SP6
ATT TAG GTG ACA CTA TAG
pGEX 3’
CCG GGA GCT GCA TGT GTC AGA GG
pGEX 5’
GGG CTG GCA AGC CAC GTT TGG TG
BGH
TAG AAG GCA CAG TCG AGG
Custom primers must be submitted at a concentration of 5pmol/µl, resuspended in Tris or dH2O (NOT EDTA)