SERVICES + PRICING

Discover more about our library preparation and sequencing services below.

Library Preparation and QC

We favour Illumina's library preparation kits, but also work with kits from other vendors and varied custom protocols. Our most common library types are listed in the pricing section below. Don't see your required library type listed? Please contact us for details. 

Note that although we currently cannot isolate input material, we can advise you on the best isolation procedures for your sample.

Process

  1. Optional: Contact us for advice and planning
  2. Consult our submission guidelines for input material
  3. Complete a sample submission form
  4. Submit sample(s) to our facility for processing

Quality Control

Sample or Library QC

Pricing is per sample, library or pool submitted. Note that our prices for library construction including incoming sample QC using fluorometric methids at no additional charge.  

Method UBC Non-UBC Academic Industry/For Profit
Qubit quantification $4 $5 $5
Agilent Bioanalyzer $12 $14 $15

 

Library Preparation

DNA Libraries

Pricing is per library and includes incoming sample quantification by fluorometric methods.

Library Type UBC Non-UBC Academic Industry/For Profit
Whole genome libraries: Illumina DNA Prep, formerly Nextera DNA Flex $70 $77 $88
Custom: Amplicon, CRISPR screens, cDNA, low input DNA, alternative protocols (e.g. NEB Next Ultra II, Nextera XT, Smart-Seq, Rhino-Seq, SparQ) Contact us Contact us Contact us

 

16S and Metagenomic Libraries

Pricing is per library and includes incoming sample quantification by fluorometric methods.

Library Type UBC Non-UBC Academic Industry/For Profit
16S rRNA gene (v3 + V4) $45 $50 $57
Shotgun metagenomic libraries: Illumina DNA Prep, formerly Nextera DNA Flex $70 $77 $88

 

RNA Libraries

Pricing is per library and includes incoming sample quantification by fluorometric methods. Agilent Bioanalyzer analysis available upon request. 

Library Type UBC Non-UBC Academic Industry/For Profit
Stranded polyA+ mRNA (Illumina TruSeq mRNA stranded) $125 $138 $157
Bacterial ribosomal depletion (NEBNext rRNA Depletion Kit, Illumina Ribo-Zero) Batch size dependent Batch size dependent Batch size dependent
Metatranscriptomic  Batch size dependent Batch size dependent Batch size dependent
Custom: Low input RNA, small RNA, total RNA, alternative protocols (e.g. NEB Next Ultra II, NEXTFLEX Combo-Seq) Contact us Contact us Contact us

 

Specialized and Custom Libraries

Pricing is per library and includes incoming sample quantification by fluorometric methods.

Library Type UBC Non-UBC Academic Industry/For Profit
Amplicon Barcoding $30 $33 $38
18S rRNA gene and ITS regions Contact us Contact Us Contact Us
Custom capture Batch size dependent Batch size dependent Batch size dependent
Tn-Seq and Bar-Seq $30 $33 $38
Pool-Seq Contact us Contact us Contact us
Custom assay development Project dependent Project dependent Project dependent

 

 

Sequencing

We accept Illumina-compatible user-prepared libraries. For such libraries, certain QC metrics are required before sample submission. Please contact us for details.  Library validation and quantification services (Qubit, BioAnalyzer, qPCR) are also available for user-prepared libraries. 

Options and Pricing

MiSeq

The MiSeq offers fast run time and long read lengths to allow assembly of small genomes or detection of target variants with unmatched accuracy. More information

The MiSeq can generate up to 25 M clusters (50 M paired-end reads) passing filter per run when using v3 kits. 

Pricing

Pricing is per run.

Version Number of Cycles Common Read Lengths UBC  Non-UBC Academic Industry/For-profit
MiSeq v2 50 1 x 50 bp $1,750 $1,825 $1,900 
MiSeq v3 150 1 x 150 bp $1,950  $2,025 $2,100
MiSeq v2 300 2 x 150 bp $2,050  $2,225 $2,200 
MiSeq v2 500 2 x 250 bp $2,450 $2,525 $2,600
MiSeq v3 600 2 x 300 bp $3,050 $3,125 $3,200 
MiSeq Micro v2 300 2 x 150 bp $1,000  $1,075 $1,150
MiSeq Nano v2 300 2 x 150 bp $800  $875 $950 
MiSeq Nano v2 300 2 x 250 bp $900 $975 $1,000 
NextSeq

The NextSeq Series provides the flexible power and simplicity you need for transcriptome and targeted resequencing. More information.

Pricing

NextSeq 500 Number of Cycles Common Read Lengths UBC  Non-UBC Academic Industry/For-profit
MId Output 150 2 x 75 bp $2,200  $2,300 $2,375 
Mid Output 300 2 x 150 bp $3,500 $3,600 $3,675 
High Output 75 1 x 75 bp $2,900 $3,000 $3,075
High Output 150 2 x 75 bp $5,200 $5,300 $5,375
High Output 300 2 x 150 bp $7,700  $7,800 $8,200 
NextSeq 2000          
P2, 400M  100 2 x 50 bp $2,500 $2,575 $2,650
P2, 400M  200 2 x 100 bp $4,500 $4,575 $4,650
P2, 400M  300 2 x 150 bp $6,000 $6,075 $6,150
P3, 1100M  50 2 x 25 bp $3,800 $3,875 $3,950
P3, 1100M 100 2 x 50 bp $5,400 $5,475 $5,550
P3, 1100M 200 2 x 100 bp $7,400 $7,475 $7,550
P3, 1100M 300 2 x 150 bp $9,800 $9,875 $9,950
HiSeq and NovaSeq

Please contact us to discuss options for sequencing your samples on HiSeq and NovaSeq instruments.   

Determining Sequencing Coverage

These links from Illumina will help you determine what coverage you need and what instrument is best suited to your project:

https://www.illumina.com/documents/products/technotes/technote_coverage_calculation.pdf

http://support.illumina.com/downloads/sequencing_coverage_calculator.html

Still unsure? CONTACT US