Discover more about our library preparation and sequencing services below.
Library Preparation and QC
We favour Illumina's library preparation kits, but also work with kits from other vendors and varied custom protocols. Our most common library types are listed in the pricing section below. Don't see your required library type listed? Please contact us for details. Prices valid until 30 June 2022.
Note that although we currently cannot isolate input material, we can advise you on the best isolation procedures for your sample.
Process
- Optional: Contact us for advice and planning
- Consult our submission guidelines for input material
- Complete a sample submission form
- Submit sample(s) to our facility for processing
Quality Control
Pricing is per sample, library or pool submitted. Note that our prices for library construction including incoming sample QC using fluorometric methods at no additional charge.
| Method | UBC | Non-UBC Academic | Industry/For Profit |
| Qubit quantification | $4 | $5 | $5 |
| Agilent Bioanalyzer | $12 | $14 | $15 |
Library Preparation
Pricing is per library and includes incoming sample quantification by fluorometric methods.
| Library Type | UBC | Non-UBC Academic | Industry/For Profit |
| Whole genome libraries: Illumina DNA Prep, formerly Nextera DNA Flex | $70 | $77 | $88 |
| Custom: Amplicon, CRISPR screens, cDNA, low input DNA, alternative protocols (e.g. NEB Next Ultra II, Nextera XT, Smart-Seq, Rhino-Seq, SparQ) | Contact us | Contact us | Contact us |
Pricing is per library and includes incoming sample quantification by fluorometric methods.
| Library Type | UBC | Non-UBC Academic | Industry/For Profit |
| 16S rRNA gene (v3 + V4) | $45 | $50 | $57 |
| Shotgun metagenomic libraries: Illumina DNA Prep, formerly Nextera DNA Flex | $70 | $77 | $88 |
Pricing is per library and includes incoming sample quantification by fluorometric methods. Agilent Bioanalyzer analysis available upon request.
| Library Type | UBC | Non-UBC Academic | Industry/For Profit |
| Stranded polyA+ mRNA (Illumina TruSeq mRNA stranded) | $125 | $138 | $157 |
| Bacterial ribosomal depletion (NEBNext rRNA Depletion Kit, Illumina Ribo-Zero) | Batch size dependent | Batch size dependent | Batch size dependent |
| Metatranscriptomic | Batch size dependent | Batch size dependent | Batch size dependent |
| Custom: Low input RNA, small RNA, total RNA, alternative protocols (e.g. NEB Next Ultra II, NEXTFLEX Combo-Seq) | Contact us | Contact us | Contact us |
Pricing is per library and includes incoming sample quantification by fluorometric methods.
| Library Type | UBC | Non-UBC Academic | Industry/For Profit |
| Amplicon Barcoding | $30 | $33 | $38 |
| 18S rRNA gene and ITS regions | Contact us | Contact Us | Contact Us |
| Custom capture | Batch size dependent | Batch size dependent | Batch size dependent |
| Tn-Seq and Bar-Seq | $30 | $33 | $38 |
| Pool-Seq | Contact us | Contact us | Contact us |
| Custom assay development | Project dependent | Project dependent | Project dependent |
Sequencing
We accept Illumina-compatible user-prepared libraries. For such libraries, certain QC metrics are required before sample submission. Please contact us for details. Library validation and quantification services (Qubit, BioAnalyzer, qPCR) are also available for user-prepared libraries. Prices valid from 1 April 2023.
Options and Pricing
The MiSeq offers fast run time and long read lengths to allow assembly of small genomes or detection of target variants with unmatched accuracy. More information
The MiSeq can generate up to 25 M clusters (50 M paired-end reads) passing filter per run when using v3 kits.
Pricing
Pricing is per run.
| Version | Number of Cycles | Common Read Lengths | UBC | Non-UBC Academic | Industry/For-profit |
| MiSeq v2 | 50 | 1 x 50 bp | $2,075 | $2,150 | $2,600 |
| MiSeq v3 | 150 | 1 x 150 bp | $2,275 | $2,350 | $2,850 |
| MiSeq v2 | 300 | 2 x 150 bp | $2,550 | $2,625 | $3,175 |
| MiSeq v2 | 500 | 2 x 250 bp | $2,925 | $3,000 | $3,650 |
| MiSeq v3 | 600 | 2 x 300 bp | $3,600 | $3,675 | $4,500 |
| MiSeq Micro v2 | 300 | 2 x 150 bp | $1,250 | $1,325 | $1,575 |
| MiSeq Nano v2 | 300 | 2 x 150 bp | $925 | $1,000 | $1,175 |
| MiSeq Nano v2 | 500 | 2 x 250 bp | $1,050 | $1,125 | $1,325 |
The NextSeq Series provides the flexible power and simplicity you need for transcriptome and targeted resequencing. More information.
Pricing
| NextSeq 500 | Number of Cycles | Common Read Lengths | UBC | Non-UBC Academic | Industry/For-profit |
| MId Output | 150 | 2 x 75 bp | $2,500 | $2,575 | $2,750 |
| Mid Output | 300 | 2 x 150 bp | $3,825 | $3,900 | $4,300 |
| High Output | 75 | 1 x 75 bp | $3,300 | $3,375 | $3,750 |
| High Output | 150 | 2 x 75 bp | $6,000 | $6,075 | $6,600 |
| High Output | 300 | 2 x 150 bp | $9,000 | $9,075 | $9,900 |
| NextSeq 2000 | |||||
| P1, 100M | 50 | 1 x 50 bp | $2,500 | $2,575 | $2,650 |
| P2, 400M | 100 | 2 x 50 bp | $2,700 | $2,775 | $2,850 |
| P2, 400M | 200 | 2 x 100 bp | $4,900 | $4,975 | $5,050 |
| P2, 400M | 300 | 2 x 150 bp | $6,500 | $6,575 | $6,650 |
| P3, 1100M | 50 | 2 x 25 bp | $4,200 | $4,275 | $4,350 |
| P3, 1100M | 100 | 2 x 50 bp | $5,900 | $5,975 | $6,150 |
| P3, 1100M | 200 | 2 x 100 bp | $8,000 | $8,075 | $8,150 |
| P3, 1100M | 300 | 2 x 150 bp | $11,000 | $11,075 | $11,150 |
Please contact us to discuss options for sequencing your samples on HiSeq and NovaSeq instruments.
Determining Sequencing Coverage
These links from Illumina will help you determine what coverage you need and what instrument is best suited to your project:
https://www.illumina.com/documents/products/technotes/technote_coverage_calculation.pdf
http://support.illumina.com/downloads/sequencing_coverage_calculator.html
Still unsure? CONTACT US