SBC provides automated fragment sizing of multicolor fluorescent PCR products, utilizing Applied Biosystems instrumentation (Applied Biosystems 3730XL 96-capillary DNA Analyzer).
Up to four different fluorescent dyes may be used for labeling, increasing throughput by multiplexing. Co-injection of an internal size standard, labeled with a different dye, provides accurate and reproducible size determination, eliminating issues of sample-to-sample or run-to-run electrophoretic variability.
Some applications which can be accommodated include:
- Microsatellite analysis
Planning your Project
Please contact SBC staff to discuss these questions:
- What fragment sizes do you expect?
- Which is the most suitable size standard?
- Will you be multiplexing? If so, which fluorophores are available to you?
Fragment Analysis kits may be purchased through Life Technologies (formerly Applied Biosystems), or researchers may choose to order customized fluorescently-labeled primers directly through SBC. Please contact SBC staff to discuss your primer labeling options.
Serial Dilution Run
In the very beginning, with the understanding that PCR conditions will remain constant throughout the project, it is essential to determine a dilution factor which is acceptable across all samples. SBC staff will generate serial dilutions of 6 random samples (10µl of each submitted), to be co-injected with the required amount of size standard. Researchers will be advised of the recommended dilution for future submissions. This complimentary serial dilution run will only be performed once for each project.
Academic Pricing - Injection Costs
To a maximum per 96-well plate
(Contact us for processing requirements)