SUBMISSION GUIDELINES

Users may submit samples for QC only, library preparation or prepared libraries for sequencing.

Process

  1. Optional: Contact us for advice and planning
  2. Consult our submission guidelines below for your service of choice
  3. Download and complete a sample submission form and email it to sequencing.centre@ubc.ca and sbc.lab@ubc.ca (cc'ing your supervisor)
  4. Once approved, submit sample(s) to our facility for processing (download instructions below)

 

Sample submission for QC only

Unless otherwise advised, please submit your DNA samples undiluted. If available, please include Nanodrop values or other quantification values in the submission form. Where possible, a minimum of 5 μL sample should be provided.

Download Sample Submission Form for DNA/RNA QC Only

 

Sample submission for library preparation

Our library construction includes incoming sample quantification, so please submit your DNA samples undiluted. If available, please include Nanodrop values or other quantification values in the submission form.

DNA should be provided in nuclease-free water or 10 mM Tris-Cl pH 8.0. The presence of EDTA can interfere with enzymatic reactions during library preparation and should therefore be avoided. A minimum of 15 μL sample should be provided.

All samples submitted for library preparation must be intact and free of contaminants. We are not responsible for poor library quality resulting from low sample quality.

Download Sample Submission Form for Library Preparation

 

Library submission for sequencing

User-prepared libraries must be compatible with the Illumina platform.

Prepared libraries should be provided at a concentration of 4 nM as determined by fluorometry or qPCR, in a minimum of 15 μl of buffer. We are able to provide library validation and quantification at extra cost.

If required, custom primers should be submitted with your library pool. We require 10 μl of primer at a concentration of 100 μM.

We are not responsible for failed or suboptimal sequencing runs resulting from poor library quality or inaccurate library quantification. Re-runs are only performed in cases of instrument failure, and although we strive to accommodate such failures in scheduling, we cannot guarantee their timing.

Download Sample Submission Form for Pool QC and/or Sequencing

  

Sample Submission Fridge

Download Submission Fridge Instructions

 


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