Users may submit samples for library preparation or user-prepared libraries for sequencing.
All fields in the appropriate sample submission form must be filled in before proceeding with any library preparation or sequencing run.
Sample submission for library preparation
All samples submitted for library preparation must be intact and free of contaminants.
Nextera XT requires 1 ng input DNA, but other library-preparation methods require higher amounts (please contact us for details).
DNA and RNA should be provided in nuclease-free water or 10 mM Tris-Cl pH 8.0. The presence of EDTA can interfere with enzymatic reactions during library preparation and should therefore be avoided. A minimum of 15 ul sample should be provided.
We require a picture of the DNA or RNA run out on a standard agarose gel with ladder, as well as an accurate quantification of concentration. DNA quantification must be based on fluorometric methods rather than nanodrop or optical density methods. We are able to provide such quantification at extra cost.
We are not responsible for poor library quality resulting from low sample quality or erroneous quantification.
Library submission for sequencing
User-prepared libraries must be compatible with the Illumina platform.
Prepared libraries should be provided at a concentration of 4 nM as determined by fluorimetry or qPCR, in a minimum of 15 ul of buffer (nuclease-free water (preferred) or 10 mM Tris-Cl pH 8.0). We are able to provide library validation and quantification at extra cost.
If required, custom primers should be submitted with your library pool. We require 10 ul of primer at a concentration of 100 uM.
We are not responsible for failed or suboptimal sequencing runs resulting from poor library quality or inaccurate library quantification. Re-runs are only performed in cases of instrument failure, and although we strive to accommodate such failures in scheduling, we cannot guarantee their timing.