The new SBC dropbox in LSI stores for easy submission of Sanger sequencing samples.
Submit your pre-mixed samples by 12:45 pm and receive your data the next business day.
Submit your samples in four easy steps:
- Prepare your premixed samples: mix your template and desired primer following the submission guidelines below.
- Log into dnaLIMS and enter your order selecting the User-Prepared Pre-Mixed Service.
- Print out your order and put it in a bag along with your samples.
- Drop in our new LSI drop-box by 12:45 pm and expect your data the next business day.
Submit template and primers premixed with water according to the table:
| Standard Template | Final Amount Required | Total Volume |
|---|---|---|
| Plasmid DNA | 400ng | 14 µl |
| purified PCR product (100 - 300 bp) | 10 ng | 14 µl |
| purified PCR product (301 - 1,000 bp) | 30 ng | 14 µl |
| purified PCR product (over 1,000 bp) | 100 ng | 14 µl |
| primer | 10 pmol | included in above |
See full details on preparing samples, template and primer requirements in our submission guidelines.
Full Service: SBC Core-Prepared: Submit your template and primers separately, as described below, and we do everything else.
Full service samples will be processed the next business day. For same-day processing, submit your samples by 9:30 am in the submission fridge in the Pharmaceutical Sciences Building (across the Agronomy Road).
| Standard Template | Concentration | Volume |
|---|---|---|
| plasmid DNA | 40 - 250 ng/µl | >10 µl per reaction |
| purified PCR product (100 - 300 bp) | 2 - 20 ng/µl | >10 µl per reaction |
| purified PCR product (301 - 1,000 bp) | 3 - 40 ng/µl | >10 µl per reaction |
| purified PCR product (over 1,000 bp) | 20 - 200 ng/µl | >10 µl per reaction |
| custom primer, if required | ||
| primer, submitted separately from template | 5 pmol/µl | >5 µl per reaction |
User-prepared, User-cleaned: Submit your cycled and cleaned reaction for electrophoresis. Refer to our guidelines for more informaiton
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