Bioinformatics Fundamentals: From Illumina Sequencer to Assessing Data Quality

Next-generation sequencing (NGS) plays an essential role in life sciences research and diagnostics. This informational session will allow you to understand the basics of generating NGS data and assessing its quality, including choosing the appropriate Illumina sequencer for your experiment, understanding the outputted sequencing data format, and understanding the report generated by the quality control tool, FastQC.

Date: November 8 & 9, 2022
Time: 3:00pm - 4:30pm
Location: Zoom 
Registration Fee: Free
System Requirements: Windows, Linux, or Mac

Agenda

Day 1:
  • General introduction to next generation sequencing (NGS)
  • Overview of Illumina sequencing platforms (MiSeq vs. NextSeq vs NovaSeq)
  • Choosing the correct sequencer for your experiment
  • Converting binary base call (BCL) sequence file to FASTQ format
  • Understanding FASTQ files
Day 2:
  • Importance of assessing data quality
  • Assessing data with FastQC
  • Understanding FasQC reports

Recordings

Recordings of the webinar can be found on VIMEO:

Day 1

Day 2

 

Please contact us if you have any questions or have suggestions for new training topics.